PROTEOMICS ANALYSIS WORKSHOP

ELECTROPHORESIS, SPECTROPHOTOMETRY AND SPECTROSCOPY BASED PROTEOMICS ANALYSIS WORKSHOP 

Assoc. Prof. Duygu ÖZEL DEMİRALP

Human Genome Project (HGP) is one of the most important projects in human history in terms of obtaining results. There are needs of new knowledge in addition to the output of HGP to develop new treatment approaches, to define relations between all disciplines and to define the cause – effect relation between all disciplines.

With the very general definition, Proteomics is the science that explains RNA-protein interaction, deals with the post-transcriptional and translational modifications in proteins in the cells and tissues.

Nowadays proteomics approaches mostly used to identify new diagnostic and therapeutic methods for the many diseases such as cancer or as a predictive purpose for some disease like epilepsy. In addition to these Proteomics can be used to identify individual therapies for the kind of complex metabolomic diseases such as obesity and diabetes. Most of the techniques that are used in proteomics is performed in our laboratory and in the scope of this course; protein isolation from different body fluids, tissues and organs, analysis of protein profile by using two dimensional gel electrophoresis (2-D PAGE), cutting spots from 2-D PAGE gels and protein identification of cutted protein spots by MALDI-TOF mass spectrometry after trypsinization will be performed. MALDI-TOF analysis results will be searched in databases such as Mascot for the purpose of protein identification by “Peptide Mass Fingerprint (PMF)” methods. Furthermore, metabolic profiling will also be performed by infrared spectroscopy in our course.

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