RNAi & miRNA WORKSHOP
PRACTICAL COURSE: RNAi APPLICATIONS and
miRNA ANALYSIS TECHNIQUES
Assist. Prof. Arzu ATALAY
MicroRNAs are tiny endogenous RNA molecules that are involved mostly in post-transcriptional gene regulation. The aim of this practical course is to silence a gene in mammalian tissue culture cell line by RNAi and observe the effects on the cell. On the other hand, an endogenous microRNA will be inactivated and expression levels will be analyzed by QRT-PCR. Different kind of RNAi and miRNA analysis techniques, application platforms, in silico tools and experimental designs will also be discussed.