Fourier Transform Infrared (FTIR) is a kind of spectroscopy method which is used to determine atomic bonds vibrations of the sample when exposed to infrared light. Therefore this technique can be used to determine small changes in the sample. The technique allow to study a wide range of samples. By means of different loading apparatus, samples in solid, liquid or gaseous phases can be studied with FT-IR spectroscopy.
Light source, interferometer and detector are the three main parts in the FT-IR. In addition to these, there are beam splitter, sample loading apparatus and computer part. The chemical bonds in the molecules are characterized by their vibration model at the specific wavelengths when they are exposed to infrared light. Thus with the analysis of their peak values, it is possible to observe changes in the molecules. In addition to that, by this technique it is possible to observe changes in secondary structure of proteins. In this context the peaks underneath the Amide I bond peak are informative.
Our unit is equipped with a “Bruker Tensor 27” (Bruker Optic GmbH, Germany) FT-IR system. The device has germanium-coated Kbr beam splitter and a high sensitivity MCT detector. The measurements are performed at the middle infrared wavelength. There are three apparatus that can be used with solid and liquid samples which are AquaSpec Cell (sensitive for protein sample that are in liquid form) and BioATR II Cell (for samples in liquid phase which allows to observe temperature depended changes in proteins). Finally the measurements analysis is performed on OPUS software.