PCR products*: 100-200bp 10-20 ng/ml
200-500bp 20-30 ng/ml
500-1000bp 30-50 ng/ml
1000-2000bp 50-60 ng/ml
> 2000bp 40-100 ng/ml
Plasmid: 100-200 ng/ml
BAC: 250 ng/ml
*Minimum 10 ul template DNA should be supplied.
** Minimum 10 ul primer (10pmol/ml) should be supplied along with template DNA.
*** PCR purification should be performed on Qiagen/Qiaquick, Promega/Wizard, MN or with an equivalent system. For PCR products less than 200 bp Exonuclease1/SAP treatment should be preferred for purification.
*****Primers should be HPLC (or OPC/Metabion or HPSF/MWG) purified. In order to get clear sequencing data the sequencing primer’s GC content, primer-dimer formation, hairpin formation criteria should be carefully considered. Applications can be made to our unit for further information on primer design.