The amount, integrity and purity of RNA samples are crucial for the quality, reliability and robustness of Real Time QRT-PCR. That is why our unit will rely on Agilent Bioanalyzer chromatogram or denaturing agarose gel profile for the evaluation of RNA samples. A260/A280 ratio of a good quality RNA sample should be between 1.9-2.1.
RNA samples should be brought to our unit on dry ice. If needed quality and quantity evaluation of RNA samples on Bioanalyzer can be performed in our unit.
It is possible to perform 50 analysis with 100 ul of cDNA synthesized from 1-2 ug of RNA sample. The RNA sample amount that should be submitted to our unit can be calculated based on this information.
Our unit can be consulted on RNA isolation methods. RNA isolation method should include a DNase treatment step.